https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25448 Wed 24 Nov 2021 15:53:38 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20669 Wed 11 Apr 2018 15:57:11 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14356 Wed 11 Apr 2018 15:41:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23916 Wed 11 Apr 2018 10:32:03 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27853 T transitions. These mutations are found throughout the melanoma genome, particularly in non-transcribed regions. The global genome repair (GGR) branch of nucleotide excision repair (NER) is responsible for repairing UV-induced DNA damage across non-transcribed and silent regions of the genome. This study aimed to examine the relationship between UVB and GGR in melanoma. DNA repair capacity and relative expression of NER in melanocytes and melanoma cell lines before and after treatment with UVB was quantified. Transcript expression from 196 melanomas was compared to clinical parameters including solar elastosis and whole transcriptome data collected. Melanoma cell lines showed significantly reduced DNA repair when compared to melanocytes, most significantly in the S phase of the cell cycle. Expression of GGR components XPC, DDB1 and DDB2 was significantly lower in melanoma after UVB. In the melanoma tumours, XPC expression correlated with age of diagnosis and low XPC conferred significantly poorer survival. The same trend was seen in the TCGA melanoma dataset. Reduced GGR in melanoma may contribute to the UV mutation spectrum of the melanoma genome and adds further to the growing evidence of the link between UV, NER and melanoma.]]> Wed 02 Mar 2022 14:27:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:7129 Sat 24 Mar 2018 08:34:10 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:4952 Sat 24 Mar 2018 07:48:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22626 Sat 24 Mar 2018 07:11:27 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:20880 Mon 11 Mar 2019 12:15:55 AEDT ]]>